Composite

Part:BBa_K1963007:Design

Designed by: Frank Sargent   Group: iGEM16_Dundee_Schools   (2016-10-09)


Device for rhamnose-dependent expression of an OsmY.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Compliant with RFC[10] for further engineering.


Source

Assembled from available Biobricks (based on natural sequneces).

References

Lange R, Barth M, Hengge-Aronis R. (1993) Complex transcriptional control of the sigma s-dependent stationary-phase-induced and osmotically regulated osmY (csi-5) gene suggests novel roles for Lrp, cyclic AMP (cAMP) receptor protein-cAMP complex, and integration host factor in the stationary-phase response of Escherichia coli. J Bacteriol 175: 7910-7917.

Steen EJ, Kang Y, Bokinsky G, Hu Z, Schirmer A, McClure A, Del Cardayre SB, Keasling JD. (2010) Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Nature 463: 559-62. doi: 10.1038/nature08721.

Le Y, Wang H. (2014) High-level soluble expression of a thermostable xylanase from thermophilic fungus Thermomyces lanuginosus in Escherichia coli via fusion with OsmY protein. Protein Expr Purif 99: 1-5. doi: 10.1016/j.pep.2014.03.004.